In this recently published journal by Wang and Cheung in the world respected journal J.AOAC International (Volume 99, Number 2, March-April 2016, pp. 539-557(19) ) a quick, easy, cheap, effective, rugged, and safe (QuEChERs) method for extraction of 187 pesticides in both red and white wine samples is presented using ultra-HPLC for the analysis of a wide range of pesticides. The pesticide residues were extracted from wine samples by a QuEChERS approach employing UCT's ENVIRO CLEAN salts/sorbents. Mylar pouches included anhydrous magnesium sulfate 6.0 g (MgSO4) and sodium acetate 1.5 g in addition to ENVIRO CLEAN 15 mL centrifuge tubes containing MgSO4 900 mg, C18 150 mg, and primary secondary amine (PSA) 300 mg for a highly productive and efficient QuEChERs clean-up.
Ultra HPLC/electrospray ionization-tandem MS quantification was achieved using matrix-matched standard calibration curves with isotopically labeled standards or a chemical analogue as internal standards with an analytical range from 5.0 to 500.0 μg/L. The method performance characteristics that included overall recovery, intermediate precision, and measurement uncertainty were evaluated according to a nested experimental design. Generally, 98.4% (in red wine) and 96.8% (in white wine) of the pesticides had recoveries between 71 and 120%; 98.9% (in red wine) and 99.5% (in white wine) of the pesticides had the intermediate precision ≤20%; and 99.5% (in red wine) and 98.4% (in white wine) of the pesticides had measurement uncertainty ≤50%. This methodology demonstrates why UCT is the premier manufacturer of QuEChERs kits and accessories.